Genetic dissection of heterosis using epistatic association mapping in a partial NCII mating design

نویسندگان

  • Jia Wen
  • Xinwang Zhao
  • Guorong Wu
  • Dan Xiang
  • Qing Liu
  • Su-Hong Bu
  • Can Yi
  • Qijian Song
  • Jim M. Dunwell
  • Jinxing Tu
  • Tianzhen Zhang
  • Yuan-Ming Zhang
چکیده

Heterosis refers to the phenomenon in which an F1 hybrid exhibits enhanced growth or agronomic performance. However, previous theoretical studies on heterosis have been based on bi-parental segregating populations instead of F1 hybrids. To understand the genetic basis of heterosis, here we used a subset of F1 hybrids, named a partial North Carolina II design, to perform association mapping for dependent variables: original trait value, general combining ability (GCA), specific combining ability (SCA) and mid-parental heterosis (MPH). Our models jointly fitted all the additive, dominance and epistatic effects. The analyses resulted in several important findings: 1) Main components are additive and additive-by-additive effects for GCA and dominance-related effects for SCA and MPH, and additive-by-dominant effect for MPH was partly identified as additive effect; 2) the ranking of factors affecting heterosis was dominance > dominance-by-dominance > over-dominance > complete dominance; and 3) increasing the proportion of F1 hybrids in the population could significantly increase the power to detect dominance-related effects, and slightly reduce the power to detect additive and additive-by-additive effects. Analyses of cotton and rapeseed datasets showed that more additive-by-additive QTL were detected from GCA than from trait phenotype, and fewer QTL were from MPH than from other dependent variables.

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عنوان ژورنال:

دوره 5  شماره 

صفحات  -

تاریخ انتشار 2015